Journal: Molecular & Cellular Oncology

Article Title: GINS1 suppresses ferroptosis by inhibiting the Hippo pathway in neuroblastoma

doi: 10.1080/23723556.2026.2715868

Figure Lengend Snippet: The impact of GINS1 on ferroptosis and the Hippo signaling pathway in neuroblastoma cells. A GO and KEGG pathway enrichment analysis of GINS1. B The ssGSEA scores of the ferroptosis pathway in Low_GINS1 and High_GINS1 groups. C The correlation between GINS1 expression and the expression of ACSL4, AIFM2, and TP53 proteins. D The gene expression in ferroptosis and Hippo pathways between neuroblastoma in Low_GINS1 and High_GINS1 groups; red boxes highlight ferroptosis genes. E The relative RFU of C11-BODIPY 581/591 were measured to assess lipid peroxidation in SH-SY5Y neuroblastoma cells transfected with shNC, shGINS1-1 and shGINS1-2. The concentration of malondialdehyde (MDA) and the iron content were also determined, respectively, as indicators of lipid peroxidation and iron metabolism. F Western blot assessed ACSL4, YAP, p -YAP, and AIFM2 in SH-SY5Y cells with shNC, shGINS1-1, and shGINS1-2. Data are presented as mean ± SD from three independent experiments, and statistical significance was determined using unpaired t-test or one-way ANOVA. The p -values are denoted by asterisks as follows: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant.

Article Snippet: Membranes were incubated with primary antibodies against ACSL4 (1:2000, Proteintech, 22401-1-AP), p -YAP (1:1000, Proteintech, 80694-2-RR), YAP (1:1000, Proteintech, 13584-1-AP), AIFM2 (1:1000, Proteintech, 20886-1-AP), p -MST1 (1:1000, Cell Signaling Technology, #3681S), MST1 (1:1000, Cell Signaling Technology, #3682S), p -LATS1 (1:1000, Cell Signaling Technology, #8654S), LATS1 (1:1000, Cell Signaling Technology, #3477S), GPX4 (1:1000, Proteintech, 67763-1-Ig), SLC7A11 (1:1000, Proteintech, 26864-1-AP) and GAPDH (1:30000, Proteintech, 60004-1-lg), followed by HRP-conjugated secondary antibodies.

Techniques: Analysis, Expressing, Gene Expression, Transfection, Concentration Assay, Western Blot

Journal: Advanced Science

Article Title: Arrayed Magnetic Accelerator Synergistically Enhances Orthodontic Tooth Movement via Microtubule Assembly‐Mediated Periodontal Remodeling

doi: 10.1002/advs.77447

Figure Lengend Snippet: SMFs amplify the response of orthodontic mechanical signaling pathways. (a) Representative immunofluorescence images of vinculin (green), F‐actin (purple), and nuclei (blue) in PDLCs under three conditions: control (without mechanical force and without SMFs), with mechanical force only, and with mechanical force plus SMFs. Scale bar: 10 µm. (b) Cell area, (c) FA area per cell, and (d) cell shape index in PDLCs. Mean ± SD. n = 24. * p < 0.05, ** p < 0.01, **** p < 0.0001. (e) FAK: Focal Adhesion Kinase. It is a mechanotransducer that becomes activated upon orthodontic force, regulating downstream bone remodeling and inflammatory responses in PDLCs. Representative immunofluorescence images of FAK (red) and nuclei (blue) in PDLCs under three conditions: control (without mechanical force and without SMFs), mechanical force only, and mechanical force plus SMFs. Scale bar: 10 µm. (f) Relative FAK integrated density per cell in PDLCs. Mean ± SD. n = 24. **** p < 0.0001. (g) Representative immunofluorescence images of YAP/TAZ (green) and nuclei (blue) in PDLCs under three conditions: control (without mechanical force and without SMFs), mechanical force only, and mechanical force plus SMFs. Scale bar: 10 µm. (h) The nuclear‐cytoplasmic ratios of YAP/TAZ in PDLCs analyzed from the immunofluorescence images. Mean ± SD. n = 24. ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: PDLCs were respectively incubated with the primary antibody for Vinculin (Proteintech, 26520‐1‐Ap, 1:300), FAK (Proteintech, 12636‐1‐AP, 1:400), YAP/TAZ (Proteintech, 13584‐1‐Ap, 1:200), α‐Tubulin (Abclonal, AC012, 1:200) and Integrin‐β1 (Abclonal, A23497, 1:200).

Techniques: Protein-Protein interactions, Immunofluorescence, Control